Lack of BRAF Mutations in Spitz nevi

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Absence of BRAF gene mutations differentiates spitz nevi from malignant melanoma.

BACKGROUND Distinction of Spitz nevus from malignant melanoma is sometimes difficult on the basis of conventional histology. A high rate of BRAF gene mutations in malignant melanomas (66%) and nevi (82%) has recently been reported. MATERIALS AND METHODS We screened a series of 20 Spitz nevi for BRAF mutations in exons 11 and 15 by denaturing gradient gel electrophoresis (DGGE). RESULTS BRAF...

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RAF proteins are serine/threonine kinases that mediate cellular responses to growth signals by activating the mitogen-activated protein kinase pathway. Mutations in the BRAF gene causing a V599E amino acid substitution that enhance the kinase activity have been described in >60% of cutaneous melanomas and premalignant melanocytic lesions. We have investigated the frequency of BRAF mutations at ...

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The Spectrum of Spitz Nevi

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ژورنال

عنوان ژورنال: Journal of Investigative Dermatology

سال: 2004

ISSN: 0022-202X

DOI: 10.1111/j.0022-202x.2004.22530.x